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The following menu user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface control NCBI Skip to main content Skip to navigation Resources How To About NCBI Accesskeys Sign in to NCBI PubMed US National Library of Medicine National Institutes of Health Search termSearch database The following autocomplete user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface controlSearch AdvancedHelp Result Filters The following popper user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface controlDisplay Settings:AbstractThe following popper user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface controlSend to: Mol Microbiol. 1989 Aug;3(8):995-1002. The terminus of the Escherichia coli chromosome is flanked by several polar replication pause sites. François V, Louarn J, Louarn JM. Source Centre de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France. Abstract Replication of two small 'constrained' regions of the Escherichia coli chromosome, one bordered by replication terminator T1 and the other by T2, displays normal velocity in the normal direction whereas it is much slower in the opposite direction (de Massy et al., 1987). The presence of multiple polar terminators has been investigated, using a bacteriophage lambda derivative which provides a replication origin movable to predetermined loci and inducible on demand. The amount of DNA made from this induced origin was determined by in vivo labelling and hybridization to probes of the surrounding region. A redundancy of terminator-like sequences, or pause sites, has been disclosed. So far, two polar pause sites, in the same orientation and separated by 50 or 80 kb, have been localized on each side of the terminus region. The results are discussed in relation to previously observations indicating that these regions are refractory to genomic inversions. PMID: 2532703 [PubMed - indexed for MEDLINE] The following toggler user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface control MeSH Terms, Substances MeSH Terms Bacteriophage lambda/genetics Base Sequence Chromosome Mapping Chromosomes, Bacterial/metabolism* DNA Replication* Escherichia coli/genetics* Immunoblotting Sequence Homology, Nucleic Acid Terminator Regions, Genetic Thymidine/metabolism Time Factors Substances Thymidine The following toggler user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface control LinkOut - more resources Supplemental Content

Save items The following setswitch user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface controlAdd to Favorites View more options Related citations in PubMed Constraints in chromosomal inversions in Escherichia coli are not explained by replication pausing at inverted terminator-like sequences. [Mol Microbiol. 1990] The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication. [Proc Natl Acad Sci U S A. 1987] Inhibition of replication forks exiting the terminus region of the Escherichia coli chromosome occurs at two loci separated by 5 min. [Proc Natl Acad Sci U S A. 1987] Review Termination of DNA replication of bacterial and plasmid chromosomes. [Mol Microbiol. 1999] Review Mechanisms of polar arrest of a replication fork. [Mol Microbiol. 2009] See reviews... See all... Cited by 10 PubMed Central articles Review Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex. [Microbiol Mol Biol Rev. 2005] Review Linkage map of Escherichia coli K-12, edition 10: the traditional map. [Microbiol Mol Biol Rev. 1998] Search for additional replication terminators in the Bacillus subtilis 168 chromosome. [J Bacteriol. 1997] See all... Related information Related Citations Compound Compound (MeSH Keyword) Substance Substance (MeSH Keyword) Cited in PMC Recent activity Clear Turn Off The terminus of the Escherichia coli chromosome is flanked by several polar repl... PubMed See more... You are here: NCBI > Literature > PubMedWrite to the Help Desk Simple NCBI Directory GETTING STARTED NCBI Education NCBI Help Manual NCBI Handbook Training & Tutorials RESOURCES Chemicals & Bioassays Data & Software DNA & RNA Domains & Structures Genes & Expression Genetics & Medicine Genomes & Maps Homology Literature Proteins Sequence Analysis Taxonomy Training & Tutorials Variation POPULAR PubMed Nucleotide BLAST PubMed Central Gene Bookshelf Protein OMIM Genome SNP Structure FEATURED Genetic Testing Registry PubMed Health GenBank Reference Sequences Map Viewer Human Genome Mouse Genome Influenza Virus Primer-BLAST Sequence Read Archive NCBI INFORMATION About NCBI Research at NCBI NCBI Newsletter NCBI FTP Site NCBI on Facebook NCBI on Twitter NCBI on YouTube NLM NIH DHHS USA.gov Copyright | Disclaimer | Privacy | Browsers | Accessibility | Contact National Center for Biotechnology Information, U.S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA The following menu user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface control NCBI Skip to main content Skip to navigation Resources How To About NCBI Accesskeys Sign in to NCBI PubMed US National Library of Medicine National Institutes of Health Search termSearch database The following autocomplete user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface controlSearch AdvancedHelp Result Filters The following popper user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface controlDisplay Settings:AbstractThe following popper user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface controlSend to: Mol Microbiol. 1989 Aug;3(8):995-1002. The terminus of the Escherichia coli chromosome is flanked by several polar replication pause sites. François V, Louarn J, Louarn JM. Source Centre de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France. Abstract Replication of two small 'constrained' regions of the Escherichia coli chromosome, one bordered by replication terminator T1 and the other by T2, displays normal velocity in the normal direction whereas it is much slower in the opposite direction (de Massy et al., 1987). The presence of multiple polar terminators has been investigated, using a bacteriophage lambda derivative which provides a replication origin movable to predetermined loci and inducible on demand. The amount of DNA made from this induced origin was determined by in vivo labelling and hybridization to probes of the surrounding region. A redundancy of terminator-like sequences, or pause sites, has been disclosed. So far, two polar pause sites, in the same orientation and separated by 50 or 80 kb, have been localized on each side of the terminus region. The results are discussed in relation to previously observations indicating that these regions are refractory to genomic inversions. PMID: 2532703 [PubMed - indexed for MEDLINE] The following toggler user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface control MeSH Terms, Substances MeSH Terms Bacteriophage lambda/genetics Base Sequence Chromosome Mapping Chromosomes, Bacterial/metabolism* DNA Replication* Escherichia coli/genetics* Immunoblotting Sequence Homology, Nucleic Acid Terminator Regions, Genetic Thymidine/metabolism Time Factors Substances Thymidine The following toggler user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface control LinkOut - more resources Supplemental Content

Save items The following setswitch user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface controlAdd to Favorites View more options Related citations in PubMed Constraints in chromosomal inversions in Escherichia coli are not explained by replication pausing at inverted terminator-like sequences. [Mol Microbiol. 1990] The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication. [Proc Natl Acad Sci U S A. 1987] Inhibition of replication forks exiting the terminus region of the Escherichia coli chromosome occurs at two loci separated by 5 min. [Proc Natl Acad Sci U S A. 1987] Review Termination of DNA replication of bacterial and plasmid chromosomes. [Mol Microbiol. 1999] Review Mechanisms of polar arrest of a replication fork. [Mol Microbiol. 2009] See reviews... See all... Cited by 10 PubMed Central articles Review Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex. [Microbiol Mol Biol Rev. 2005] Review Linkage map of Escherichia coli K-12, edition 10: the traditional map. [Microbiol Mol Biol Rev. 1998] Search for additional replication terminators in the Bacillus subtilis 168 chromosome. [J Bacteriol. 1997] See all... Related information Related Citations Compound Compound (MeSH Keyword) Substance Substance (MeSH Keyword) Cited in PMC Recent activity Clear Turn Off The terminus of the Escherichia coli chromosome is flanked by several polar repl... PubMed See more... You are here: NCBI > Literature > PubMedWrite to the Help Desk Simple NCBI Directory GETTING STARTED NCBI Education NCBI Help Manual NCBI Handbook Training & Tutorials RESOURCES Chemicals & Bioassays Data & Software DNA & RNA Domains & Structures Genes & Expression Genetics & Medicine Genomes & Maps Homology Literature Proteins Sequence Analysis Taxonomy Training & Tutorials Variation POPULAR PubMed Nucleotide BLAST PubMed Central Gene Bookshelf Protein OMIM Genome SNP Structure FEATURED Genetic Testing Registry PubMed Health GenBank Reference Sequences Map Viewer Human Genome Mouse Genome Influenza Virus Primer-BLAST Sequence Read Archive NCBI INFORMATION About NCBI Research at NCBI NCBI Newsletter NCBI FTP Site NCBI on Facebook NCBI on Twitter NCBI on YouTube NLM NIH DHHS USA.gov Copyright | Disclaimer | Privacy | Browsers | Accessibility | Contact National Center for Biotechnology Information, U.S. National Library of Medicine 8600 Rockville Pike, Bethesda MD, 20894 USA

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