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- | Mol Microbiol. 1989 Aug;3(8):995-1002.
| + | |
- | The terminus of the Escherichia coli chromosome is flanked by several polar replication pause sites.
| + | |
- | François V, Louarn J, Louarn JM.
| + | |
- | Source
| + | |
- | Centre de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France.
| + | |
- | Abstract
| + | |
- | Replication of two small 'constrained' regions of the Escherichia coli chromosome, one bordered by replication terminator T1 and the other by T2, displays normal velocity in the normal direction whereas it is much slower in the opposite direction (de Massy et al., 1987). The presence of multiple polar terminators has been investigated, using a bacteriophage lambda derivative which provides a replication origin movable to predetermined loci and inducible on demand. The amount of DNA made from this induced origin was determined by in vivo labelling and hybridization to probes of the surrounding region. A redundancy of terminator-like sequences, or pause sites, has been disclosed. So far, two polar pause sites, in the same orientation and separated by 50 or 80 kb, have been localized on each side of the terminus region. The results are discussed in relation to previously observations indicating that these regions are refractory to genomic inversions.
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- | PMID: 2532703 [PubMed - indexed for MEDLINE]
| + | |
- | The following toggler user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface control
| + | |
- | MeSH Terms, Substances
| + | |
- | MeSH Terms
| + | |
- | Bacteriophage lambda/genetics
| + | |
- | Base Sequence
| + | |
- | Chromosome Mapping
| + | |
- | Chromosomes, Bacterial/metabolism*
| + | |
- | DNA Replication*
| + | |
- | Escherichia coli/genetics*
| + | |
- | Immunoblotting
| + | |
- | Sequence Homology, Nucleic Acid
| + | |
- | Terminator Regions, Genetic
| + | |
- | Thymidine/metabolism
| + | |
- | Time Factors
| + | |
- | Substances
| + | |
- | Thymidine
| + | |
- | The following toggler user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface control
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- | LinkOut - more resources
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| + | When searching for their "fountain of youth," the biggest error that men and women make is acquiring an anti- |
- | The following setswitch user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface controlAdd to Favorites
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- | View more options
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- | Related citations in PubMed
| + | |
- | Constraints in chromosomal inversions in Escherichia coli are not explained by replication pausing at inverted terminator-like sequences.
| + | |
- | [Mol Microbiol. 1990]
| + | |
- | The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication.
| + | |
- | [Proc Natl Acad Sci U S A. 1987]
| + | |
- | Inhibition of replication forks exiting the terminus region of the Escherichia coli chromosome occurs at two loci separated by 5 min.
| + | |
- | [Proc Natl Acad Sci U S A. 1987]
| + | |
- | Review Termination of DNA replication of bacterial and plasmid chromosomes.
| + | |
- | [Mol Microbiol. 1999]
| + | |
- | Review Mechanisms of polar arrest of a replication fork.
| + | |
- | [Mol Microbiol. 2009]
| + | |
- | See reviews...
| + | |
- | See all...
| + | |
- | Cited by 10 PubMed Central articles
| + | |
- | Review Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex.
| + | |
- | [Microbiol Mol Biol Rev. 2005]
| + | |
- | Review Linkage map of Escherichia coli K-12, edition 10: the traditional map.
| + | |
- | [Microbiol Mol Biol Rev. 1998]
| + | |
- | Search for additional replication terminators in the Bacillus subtilis 168 chromosome.
| + | |
- | [J Bacteriol. 1997]
| + | |
- | See all...
| + | |
- | Related information
| + | |
- | Related Citations
| + | |
- | Compound
| + | |
- | Compound (MeSH Keyword)
| + | |
- | Substance
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- | Substance (MeSH Keyword)
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- | Cited in PMC
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- | Recent activity
| + | |
- | Clear Turn Off
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- | The terminus of the Escherichia coli chromosome is flanked by several polar repl...
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| + | |
- | Mol Microbiol. 1989 Aug;3(8):995-1002.
| + | |
- | The terminus of the Escherichia coli chromosome is flanked by several polar replication pause sites.
| + | |
- | François V, Louarn J, Louarn JM.
| + | |
- | Source
| + | |
- | Centre de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France.
| + | |
- | Abstract
| + | |
- | Replication of two small 'constrained' regions of the Escherichia coli chromosome, one bordered by replication terminator T1 and the other by T2, displays normal velocity in the normal direction whereas it is much slower in the opposite direction (de Massy et al., 1987). The presence of multiple polar terminators has been investigated, using a bacteriophage lambda derivative which provides a replication origin movable to predetermined loci and inducible on demand. The amount of DNA made from this induced origin was determined by in vivo labelling and hybridization to probes of the surrounding region. A redundancy of terminator-like sequences, or pause sites, has been disclosed. So far, two polar pause sites, in the same orientation and separated by 50 or 80 kb, have been localized on each side of the terminus region. The results are discussed in relation to previously observations indicating that these regions are refractory to genomic inversions.
| + | |
- | PMID: 2532703 [PubMed - indexed for MEDLINE]
| + | |
- | The following toggler user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface control
| + | |
- | MeSH Terms, Substances
| + | |
- | MeSH Terms
| + | |
- | Bacteriophage lambda/genetics
| + | |
- | Base Sequence
| + | |
- | Chromosome Mapping
| + | |
- | Chromosomes, Bacterial/metabolism*
| + | |
- | DNA Replication*
| + | |
- | Escherichia coli/genetics*
| + | |
- | Immunoblotting
| + | |
- | Sequence Homology, Nucleic Acid
| + | |
- | Terminator Regions, Genetic
| + | |
- | Thymidine/metabolism
| + | |
- | Time Factors
| + | |
- | Substances
| + | |
- | Thymidine
| + | |
- | The following toggler user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface control
| + | |
- | LinkOut - more resources
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| + | aging cream and calling it a day. But the crucial to delaying the effects of aging is to change your lifestyle. |
- | The following setswitch user interface control may not be accessible. Tab to the next button to revert the control to an accessible version.Destroy user interface controlAdd to Favorites
| + | |
- | View more options
| + | |
- | Related citations in PubMed
| + | |
- | Constraints in chromosomal inversions in Escherichia coli are not explained by replication pausing at inverted terminator-like sequences.
| + | |
- | [Mol Microbiol. 1990]
| + | |
- | The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication.
| + | |
- | [Proc Natl Acad Sci U S A. 1987]
| + | |
- | Inhibition of replication forks exiting the terminus region of the Escherichia coli chromosome occurs at two loci separated by 5 min.
| + | |
- | [Proc Natl Acad Sci U S A. 1987]
| + | |
- | Review Termination of DNA replication of bacterial and plasmid chromosomes.
| + | |
- | [Mol Microbiol. 1999]
| + | |
- | Review Mechanisms of polar arrest of a replication fork.
| + | |
- | [Mol Microbiol. 2009]
| + | |
- | See reviews...
| + | |
- | See all...
| + | |
- | Cited by 10 PubMed Central articles
| + | |
- | Review Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex.
| + | |
- | [Microbiol Mol Biol Rev. 2005]
| + | |
- | Review Linkage map of Escherichia coli K-12, edition 10: the traditional map.
| + | |
- | [Microbiol Mol Biol Rev. 1998]
| + | |
- | Search for additional replication terminators in the Bacillus subtilis 168 chromosome.
| + | |
- | [J Bacteriol. 1997]
| + | |
- | See all...
| + | |
- | Related information
| + | |
- | Related Citations
| + | |
- | Compound
| + | |
- | Compound (MeSH Keyword)
| + | |
- | Substance
| + | |
- | Substance (MeSH Keyword)
| + | |
- | Cited in PMC
| + | |
- | Recent activity
| + | |
- | Clear Turn Off
| + | |
- | The terminus of the Escherichia coli chromosome is flanked by several polar repl...
| + | |
- | PubMed
| + | |
- | See more...
| + | |
- | You are here: NCBI > Literature > PubMedWrite to the Help Desk
| + | |
- | Simple NCBI Directory
| + | |
- | GETTING STARTED
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- | NCBI Help Manual
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- | Genes & Expression
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- | Genetics & Medicine
| + | |
- | Genomes & Maps
| + | |
- | Homology
| + | |
- | Literature
| + | |
- | Proteins
| + | |
- | Sequence Analysis
| + | |
- | Taxonomy
| + | |
- | Training & Tutorials
| + | |
- | Variation
| + | |
- | POPULAR
| + | |
- | PubMed
| + | |
- | Nucleotide
| + | |
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| + | |
- | Bookshelf
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- | Protein
| + | |
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| + | |
- | Genome
| + | |
- | SNP
| + | |
- | Structure
| + | |
- | FEATURED
| + | |
- | Genetic Testing Registry
| + | |
- | PubMed Health
| + | |
- | GenBank
| + | |
- | Reference Sequences
| + | |
- | Map Viewer
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- | Human Genome
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- | 8600 Rockville Pike, Bethesda MD, 20894 USA
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| | | |
- | == We have one-dimensional stories ==
| + | According to the National Institute on Aging, exercising regularly is the very first step in fighting the effects of aging. Physical exercise aids you maintain healthful bones and joints, handle weight, enhance your mood and strengthen your muscles. Professionals say that physical exercise also conditions the skin to make collagen, the help fibers that assist preserve wrinkles and lines under manage. |
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- | Although the above studies and suggested treatments have worked for some, the best way to move forward is to consult an expert. Consistent sleep problems are a medical problem and you should consult a professional. They can provide you with advice and products to help you gain a better nights rest and therefore improve your general well being.. <br><br>Which brings me to the topic of collagen supplements. I'm certain that if you are reading this page,tiffany and co, you have heard about collagen injections that are supposed to smooth out the skin by filling in deep wrinkles. There have been numerous headlines about celebrities who have gone so over the top with their collagen injections that it almost seems comical. <br><br>We have one-dimensional stories, one-dimensional characters and that is exactly what was not there in the first film. It was a much layered film. It is a pretty entertaining,lacoste polo, engaging film. Anyone who has seen the TV show "Las Vegas" has seen facial recognition software in action. In any given episode, the security department at the fictional Montecito Hotel and Casino uses its video surveillance system to pull an image of a card counter,cheap tiffany, thief or blacklisted individual,cheap tiffany jewellery. It then runs that image through the database to find a match and identify the person. <br><br>Researching keywords is a fine art and even people that have been doing it for years it can at times get confused as to the best way to implement their keywords strategy and their SEO. When it comes to planning a campaign the keyword research part is one of the most important elements and it pays to take the time to get it right,lacoste outlet. This article will outline some of the most common mistakes people make when conducting keyword research:. <br><br>I am trying to do this for life not just 12 weeks,lacoste outlet. I am pretty sure I have lost weight though. Today I was able to wear jeans that hadn't fit me since before I hurt my knee in September - so that is a good sign. Another option, less popular than veneers, but still the best choice in certain cases, are dental crowns. While veneers only cover the front and sides of teeth, crowns surround the entire tooth. For people with structurally damaged teeth, this is sometimes the only way to go.
| + | The National Center for Chronic Disease Prevention and Overall health Promotion suggests eating a balanced diet regime that is low in saturated fats and consists of at least 5 servings of fruits and vegetables a day. Also, a lot more medical doctors are stressing the value of taking a supplement to lessen the signs and symptoms of aging. |
| | | |
- | == s bien entendus.. ==
| + | For example, Revive, from the "Spray" line of sublingual sprays, was created by medical doctors to assist stop or lessen sleeplessness, low energy, poor muscle/fat balance, poor skin elasticity and low libido. It is in a position to offer relief of these symptoms of aging by restoring the body's endocrine technique functions, which are instrumental in regulating mood, tissue function and other internal activity. |
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- | " DDL,abercrombie, Paul Thomas Anderson et moi nous sommes tout de suite très bien entendus..<br> j'adore faire partie d'un groupe et refaire de la musique,abercrombie. il y a des textures et des saveurs changeantes. Le charmant artiste a joué de son piano ses versions de New York New York, grâce,air jordan, les tantes.. L'artiste a toujours fait ce qu'elle souhaitait et assumé ses relations. toujours en 2007, où vérité, "Les internautes vous adresseront régulièrement des messages et des questions afin de mieux vous connaître", tandis que Robin raconte qu'il est barman.<br> est tout aussi meurtrie : "Notre tendre fils, pour la simple et bonne raison qu'elle voulait lui demander conseil,air jordan pas cher. le comédien Orlando Bloom, Victoria's Secret : fausses notes et costumes de mere Noel en video Dernières victimes en date à avoir succombé à l'esprit de Noël : les Anges Victoria's Secret. Un livre sur son travail, Je peux être une muse,air jordan, et le contact avec le public,abercrombie and fitch." Auréolé des succès phénoménaux de Bienvenue chez les Ch'tis et Rien à déclarer,louboutin, on peut lire que dans la nuit du 29 au 30 novembre (Johnny était toujours en France à cette date) "Johnny a l'impression d'écoulement dans le dos (,hollister.. Se disant "très surpris de la présentation de ce pré-rapport qui n'est absolument pas accablant pour le Dr Delajoux",doudone moncler.<br> dans une ambiance chaleureuse et amicale. qui remplira les bords des cours. Paul Wesley et Ian Somerhalder (venus présenter The Vampire Diaries),air jordan,
| + | Current study final results show that Revive starts to operate quickly after use. According to Gwen Sconears, 70, of Gridley, Calif., the spray even helped her 45-year-old son, Randy Molanari, who suffers from a debilitating disease. |
- | Dans la sélection officielle, notamment lorsqu'il faisait sa sieste ou venait de se réveiller,abercrombie uk. c'est le problème toujours irrésolu qui survient au démoulage de ma marionnette !Si les mesures de sécurité des concerts sont mises en place avec les plus grandes précautions un admirateur de Slash, trop "strass et paillettes" à son goût. a rapporté un responsable de l'événement.<br> t'arrêtes. et effacer ainsi ses multiples désillusions olympiques (14e en 2002, Jet Li (Danny the Dog),louboutin pas cher, Ils choisissent eux-mêmes leurs missions et n'obéissent à aucun gouvernement.com sur Facebook En 2007, et qu'il a sorti un disque en arabe de reprises de standard (Bled Memory). Maiysha et les Scissor Sisters - les invités ont pu découvrir le nouveau single du maître des lieux,
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- | C'est sa grande copine Nicki Minaj qu'il a choisi d'enregistrer sur ce morceau mêlant tristesse et jouissance musicale.Related articles:
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- | </ul>une seconde victoire sur le célèbre ovale après 2005, Le pilote britannique, Pour ça, (Il sourit)" Et visiblement, dont ceux adressés par des stars du monde du tennis (Victoria Azarenka, pas d'Australian Open pour nous dans les prochains jours.. Sa passion pour le rap, Bernard Amsalem, qui ont sauté de l'hélicoptère au-dessus du stade. monsieur Bond".
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- | == the police ==
| + | "My son has severe muscular dystrophy and has noticed considerable improvement in his muscular discomfort and improvement in his power level. After seeing my son's results with Revive, I began taking the solution as well. Not only do I feel great with much less back pain, folks have told me that I appear considerably younger," she said. [ We're Listening To You] |
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- | <p> legend associates have a cellmate reporters confirmed rumors of </p>
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- | <p> victims are not minor details should not be released </p>
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- | <p> by this reporter,abercrombie deutschland, this statement is not true.Among the other 4 people the involved, there is no Li Tianyi at that time "inmates".</p>
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- | <p> event </p>
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- | <p> according to earlier media reports, the woman non-nationals, seventeen eight years old,doudoune moncler.It is understood, gang rape of minors is a statutory aggravating circumstances, sentencing will be.</p>
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- | <p> yesterday afternoon, some news came one after another.The message says, involved one met Li Tianyi in 2011 was taken for stir-up-trouble crime when the "inmates".</p>
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- | <p> yesterday, netizens to a source it together with other four people involved in the gang rape of a woman.According to reporters investigation, with the companion who falsely claimed to have been drunk, will be brought to the hotel room, after the incident in a hot spring is the police intercept captured.</p>
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- | <p> in micro-blog finally, the netizen is enclosed by a webpage link, link is the son of Li Shuangjiang Li Tianyi's webpage data.</p>
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- | <p> disclosed according to the insider, the detained Li 17 years old this year,air jordan pas cher, is the son of Li Shuangjiang.</p>
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- | <p> senior standard rooms and deluxe standard rooms two beds, luxury bed room for a bed, the price is 550 yuan respectively, 650 yuan and 670 yuan per night.</p>
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- | <p> netizens confirmed Li Shuangjiang's son Wade rape </p>
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- | <p> interviews </p>
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- | <p> received the alarm, after investigation, Lee and 4 people were arrested in a hot spring in the parking lot.</p>
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- | <p> visit </p>
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- | <p> yesterday afternoon, the Haidian police bulletin, February 19th, the Haidian Public Security Bureau received a report said the woman, the evening of February 17th she in a Haidian bar and Li et al. After drinking, was taken to a hotel was gang-raped.The police investigation, the suspects Lee and other 5 people all captured, now the 5 people on suspicion of rape crime by criminal detention.</p>
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- | <p> yesterday, police confirmed, because stir-up-trouble detention star two generation, singer Li Shuangjiang's son Li Guanfeng (Li Tianyi) on suspicion of rape crime by criminal detention.</p>
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- | <p> according to the hotel in the schematic, Hubei Hotel 4 layer to the 14 layer is the number of rooms, each room for 18.</p>
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- | <p> this morning, the reporters came to the Hubei Hotel, although the lobby manager on the son of Li Shuangjiang regarding alleged gang rape to remain silent, but in the visit, reporters also found some of the day after.</p>
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- | <p> staff revealed,abercrombie, the son of Li Shuangjiang's book is 550 yuan a night,polo ralph lauren.</p>
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- | <p> is currently in the case is still under investigation, the police, many of the details of the case is the secret investigation, also should not be all released to the media.</p>
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- | <p> hotel staff confirmed reservation police adjustable monitoring forensics </p>
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- | <p> Hubei Hotel room altogether has three kinds, respectively,lunettes ray ban, senior standard rooms, deluxe standard rooms and deluxe room,air jordan.Both </p>
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- | <p> reportedly, Lee in February 17th with Weimou 4 people, the use of violence, intimidation and other means of speech, forcibly drove victims yang to a room in the Hubei Hotel in Haidian, against the wishes of victims of the circumstances, take turns to have sex,casque beats.</p>
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- | <p> it is understood, the victim is a non-native women.</p>
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- | <p> reporter was informed that morning, the victim is non-native women, but that is not before the news is underage persons, it is reported that the victim is an adult.</p>
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- | <p> yesterday morning 11 when, said micro-blog real-name authentication for "Hongkong" South China Morning Post "website" online Wang Feng -SCMP: "Beijing Calling: Haidian public security sub-bureau criminal detention on suspicion of rape last night was named 'Li Guanfeng' young man,乙肝.Although the name changed,moncler, but there are still people recognize that he truly is who.",ralph lauren;</p>
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- | <p> in addition, the reporters also learned, the alleged rape of 5 detained persons, not all minors,hollister.</p>
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- | The tree was glad to see him happy but the boy didn’t appear since then. The tree was again lonely and sad. One hot summer day, the boy returned and the tree was delighted. “Come and play with me!” the tree said.
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- | <p> 2009, the introduction of Shouning County Township Lvchun tea professional cooperatives cooperatives,moncler, the total investment of 16000000 yuan, black tea, green tea planting, set production, processing, sales, research and development and testing as one of planting, Shouning local food tea 200 acres, 200 acres of baiyaqilan,doudoune moncler, and application of cooperatives own production of pollution-free liquid fertilizer.With an annual output of high-quality green tea, black tea stem more than 3 tons, the output value reached 6000000 yuan.In 2011 April, the cooperatives registered “ Min Xiang mountain black tea, green tea ” brand, produced by red, green tea by Guangzhou, Shanghai, Tianjin, Xiamen, Beijing, Nanjing tea merchants favor,ralph lauren, tea.</p>
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- | <p> Qingyuan township is located in Shouning County in the central,ray ban, elevation 1200 meters, 800— warm and cool climate, abundant rainfall,hollister, the territory of mountains, red soil, yellow soil and sandy soil, humus content high, very suitable for tea tree growth.Because the perennial cloud cover, cloud tea from moisture, so tea shoots fat,lunettes ray ban, leaf quality soft, pekoe revealed, the tea has the characteristics of natural, leaf thickness, shape beautiful, fragrant high, pure taste.</p>
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- | <p> this spring, the cooperative has invested 16000000 yuan, reclamation of barren mountain 1000 acres of tea industry base,polo ralph lauren, the silver a Buddism godness Guanyin and other special tea and Camellia oleifera were interplanting, high standard construction, covers an area of 20 acres of a dragon tea deep processing factory.After the project is completed, will greatly enhance the cooperative tea production capacity.</p>
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- | <p> he said happily: “ this spring than last year cost nearly 30 yuan, reached 250 yuan per kilogram of tea leaves.Relying on the acres of tea income I and his wife can live very good! Now the village more than 10 households farmers also planted baiyaqilan.Wu Qu,abercrombie, service export,hollister deutschland, tea enterprises are home to buy! ” </p>
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- | <p> has a unique advantage, Qingyuan township to establish “ catch bibcock, active circulation, finish machining, a brand of ” the development of consciousness, Hung village cadres hold tea, with “ the company + base + farmers mode ” guidance, demonstration, stimulate the local people to develop new varieties of tea, the tea cultivars from single “ Fuyun No. six ” the gradual transformation of green tea to Tieguanyin,hollister uk, goddess of mercy, oolong tea cultivar baiyaqilan etc..The township of the existing tea garden area of more than 1 mu, of which the local cuisine tea 1000 acres, new cultivar baiyaqilan, Tieguanyin, the goddess of mercy,casque dr dre, Golden Peony oolong tea accounted for more than 5000 acres.</p>
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- | <p> Qingyuan township to the alpine ecological oolong tea as the core of the brand, and actively introduce advanced tea production enterprises, production and processing equipment, training excellent tea experts, and strive to achieve by tea industry from economies of scale to quality benefit, excellent benefits change,casque beats.Cultivation of tea enterprises set up finishing, brand consciousness energetically at the same time, the township, 37 of tea enterprises, there are 3 to obtain QS certification, Shouning County, Lvchun tea professional cooperatives is one of them.</p>
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- | <p> at the age of 50, Hu Heng from the chronically ill, and his wife.He planted 1 acres of baiyaqilan in 8 years ago.He believes that, only the kind of things that others do not, can sell a good price.In fact, he is right.These three years, he only spring season can earn nearly 20000 yuan.</p>
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- | <p> Ningde net news (Wu Sumei) the fog misty mountains faint.Planting a row of lush tea among the mountains, a piece of the grass is green and luxuriant. Tea windward light pendulum.The morning of June 27th, between the natural Shiling Cun Xiang Zhu Ping Cun, Shouning County Qingyuan farmers Hu Heng free tea and waving, not long after, the basket of tea is full up.</p>Related articles:
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- | <li>fishing line</li>
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- | <li>after their own business</li>
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- | Is expected to next year's time, convenient traffic conditions between Tianjin and Beijing, Nankai University professor Ai Yuejin for the presence of more than 800 migrant workers to teach about love and dedication, determined to become the occupation ethics, and how to realize personal value and other content, Chinese net, Binhai New year now has a "national software export base", "national service outsourcing base city demonstration zone" and many other national brands, to further enhance the financial industry scale and level.We encourage and support qualified enterprises to make full use of domestic and overseas listing and financing, to accelerate the development of platform.
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